Having just been to a conference, I saw a lot of different presentations, and saw a few things that [a] I hope I don't do when I'm presenting, and [b] definitely didn't help the speaker's cause. The biggest one was the number of speakers who kept turning back to look at there slides on the big screen. Not just a little peek to make sure they were in the right spot and get their bearings. I mean a full on reading of the slide with their back to us. Well, not reading - I don't think anybody pulled that old stunt. But looking at their talking points and keeping their back to us for the most part. Definitely not a very positive experience - at least some of them had content that made it possible to overlook this, but...
The other problem, one guy (won't mention any names) had his slides on a timer, so he could walk around. The problem was, his time interval was set too short, and he constantly had to go to his computer an re-adjust. If you want to walk around (and I think that's a great thing - I need to figure out how to do it), get one of those wireless remote things to control your slides.
On the positive side, several of the speakers were very obviously letting their true selves show, and it made the presentation much better. I don't know how they were able to be so relaxed, other than experience, which I'm sure was the case, but they were joking around, even poking fun at themselves, and just generally very engaging. I know its a hard skill to acquire - I am certain I'm not there yet, but its worth the practice, because these guys came across as real pros.